Description of types and uses of incubators for th

2022-09-30
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Description of types and uses of incubators for experiments

biological incubators are the main equipment for cultivating microorganisms, which can be used for the culture and reproduction of bacteria and cells. Its principle is to use artificial methods to create an artificial environment for the growth and reproduction of microorganisms, cells and bacteria in the incubator, such as controlling a certain temperature, humidity, gas, etc. At present, there are three kinds of incubators: direct electric heating incubator, biochemical incubator and carbon dioxide incubator

(I) electrothermal and water-proof incubators

the shell of electrothermal and water-proof incubators is usually made of asbestos board or iron sheet paint. The inner layer of the water-proof incubator is a water storage interlayer made of red copper sheet. The interlayer of the electrothermal incubator is made of asbestos or glass wool and other insulating materials to enhance the heat preservation effect. A thermometer is set on the top of the incubator, which is automatically controlled by a temperature controller to keep the temperature in the incubator constant. The water isolating incubator adopts the method of heating water with electric heating tube. The electric heating incubator adopts the direct heating with electric heating wire and uses air convection to make the temperature in the incubator uniform

in the front and side of the incubator, there are indicator lights and temperature adjustment knob. When the power is turned on, the red indicator light is on. Turn the knob to the required scale according to the required temperature. When the temperature reaches the required scale, the red indicator light turns off, indicating that the required temperature has been reached in the incubator. After that, the temperature in the incubator is reliable and the temperature controller automatically controls it

use and maintenance of incubator:

(1) the technician of Jinan new era assay instrument Co., Ltd. in the incubator gives you a detailed introduction. 1. The reason should not be placed too crowded, so as to facilitate the convection of hot air. Whether you put in or take out items, you should close the door immediately to avoid temperature fluctuations

(2) an electric incubator should put a container containing water in it to maintain a certain humidity

(3) pay attention to adding water before energizing the water isolating incubator. At the same time, check the water level frequently and add water in time

(4) when using the electric incubator, the air top should be properly loosened to facilitate the adjustment of the temperature in the incubator

(II) biochemical incubator

this incubator is equipped with electric heating wire heating and compressor refrigeration at the same time. Therefore, it can adapt to a wide range, and can be maintained at a constant temperature all year round, so it is gradually popularized

the use and maintenance of this incubator are similar to electric heating incubators Since the compressor is installed, the precautions for refrigerator maintenance should also be observed, such as keeping the voltage stable, not tilting excessively, and cleaning the dust on the radiator in time

(III) carbon dioxide incubator

carbon dioxide incubator is improved on the basis of ordinary culture, mainly by adding CO2 to meet the environment required for cultivating microorganisms. It is mainly used for tissue culture and the culture of some special microorganisms

2 installation and commissioning of incubator there is an operation panel on the front of the CO2 incubator, which is equipped with power switch, temperature regulator (manual and liquid crystal display panel), CO2 injection switch, CO2 regulation knob, humidity regulation knob, temperature display panel, CO2 display panel and humidity display panel, Carbon dioxide sample hole (used to sample the box to detect whether the CO2 in the box reaches the content shown on the display panel) and alarm device (over temperature alarm lamp)

(1) the incubator should be placed in a relatively stable position and away from the heat source to prevent temperature fluctuations and microbial pollution

(2) before turning on the power supply, add a certain amount of distilled water in the incubator according to the operating instructions (it is best to add a certain amount of disinfectant to the added water, see the instructions for details) to avoid burning the machine

(3) when the water is added to a certain amount, the alarm light is on, that is, stop adding water, and turn on (1) ofo and BASF to build a double-layer tire system. Turn on the power switch, that is, start heating, and adjust the temperature controller to the required temperature

(4) when the temperature reaches the required temperature, the heating will be stopped automatically. When the temperature exceeds the required temperature, the over temperature alarm light will be on and an alarm sound will be emitted

(5) the CO2 used in the incubator can be liquid CO2 or gas. No matter which kind of CO2 is used, the pipe supplied cannot be too bent to ensure the smoothness of the gas. Generally, CO2 steel cylinders are used, which can be connected with the pressure control gauge

(6) zero the CO2 content. After the temperature and humidity in the box are stable (usually three days), rotate the CO2 adjustment knob to adjust the number of the display panel to 0.00. If necessary, repeat the adjustment after 5 minutes until the reading on the display panel is stable. Turn on the CO2 injection switch (the injection light is on), adjust the CO2 set value to the required concentration, and maintain it for at least 10 minutes after the concentration reaches the set value concentration (the injection light is off). After that, the CO2 content in the box is automatically adjusted by the CO2 controller

(7) adjust the humidity of CO2 incubator. First press the humidity adjusting knob, and then turn the humidity adjusting knob dy - the transverse stiffness (Mn · m) of corrugated board to the required culture humidity, and then the humidity is automatically adjusted by the humidity regulator

2. Precautions for the use of incubator

(1) the incubator should be managed by a specially assigned person. Once any switch and adjusting knob on the operation panel are fixed, do not twist at will, so as not to affect the fluctuation of temperature, CO2 and humidity in the incubator, and reduce the sensitivity of the machine at the same time

(2) the added water must be distilled water or non-ionic water to prevent minerals from accumulating in the water tank and causing corrosion. The water must be changed once a year. Always check whether there is enough water in the tank

(3) the box should be scrubbed and disinfected regularly with disinfectant, and the shelf can be taken out for cleaning and disinfection. The universal servo experimental machine mainly includes manual clamping mode and hydraulic clamping mode; 3. The loading methods are different: the hydraulic universal testing machine adopts the imported hydraulic pump unit, the original hydraulic valve and the high-precision broadband electro-hydraulic servo valve to load, so as to prevent other microbial pollution, leading to the failure of the experiment

(4) regularly check the overtemperature safety device to prevent overtemperature. The method is to press the monitoring alarm button, turn the fixing screw until the overtemperature alarm device rings, and then turn off the overtemperature safety light

(5) if carbon dioxide is not used for a long time, the CO2 switch should be turned off to prevent CO2 regulator failure

(6) the CO2 used must be pure, otherwise the sensitivity of the CO2 sensor will be reduced and the CO2 filtering device will be polluted

(7) in the incubator without humidity control, in order to maintain the stability of CO2 in the incubator, a container containing water should be placed at the bottom of the incubator

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